PoplarV1, Main, Exploration, bibRecord, 000202

Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.

Identifieur interne : 000202 ( Main/Exploration ); précédent : 000201; suivant : 000203

Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.

Auteurs : Shijing Xu [République populaire de Chine] ; Yiqiu Zhang [République populaire de Chine] ; Shengchun Li [République populaire de Chine] ; Ling Chang [République populaire de Chine] ; Yuyong Wu [République populaire de Chine] ; Jiang Zhang [République populaire de Chine]

Source :

Plant cell reports [ 1432-203X ] ; 2020.

RBID : pubmed:31797051

Descripteurs français

KwdFr :
- Animaux (MeSH), Bacillus thuringiensis (métabolisme), Coléoptères (effets des médicaments et des substances chimiques), Coléoptères (physiologie), Comportement alimentaire (effets des médicaments et des substances chimiques), Endotoxines (métabolisme), Endotoxines (toxicité), Feuilles de plante (parasitologie), Génome plastidique (MeSH), Hémolysines (métabolisme), Hémolysines (toxicité), Larve (effets des médicaments et des substances chimiques), Larve (physiologie), Phénotype (MeSH), Plastes (métabolisme), Populus (génétique), Populus (parasitologie), Protéines bactériennes (métabolisme), Protéines bactériennes (toxicité), Transformation génétique (MeSH), Vecteurs génétiques (métabolisme), Végétaux génétiquement modifiés (MeSH).
MESH :
- effets des médicaments et des substances chimiques : Coléoptères, Comportement alimentaire, Larve.
- génétique : Populus.
- métabolisme : Bacillus thuringiensis, Endotoxines, Hémolysines, Plastes, Protéines bactériennes, Vecteurs génétiques.
- parasitologie : Feuilles de plante, Populus.
- physiologie : Coléoptères, Larve.
- toxicité : Endotoxines, Hémolysines, Protéines bactériennes.
- Animaux, Génome plastidique, Phénotype, Transformation génétique, Végétaux génétiquement modifiés.

English descriptors

KwdEn :
- Animals (MeSH), Bacillus thuringiensis (metabolism), Bacterial Proteins (metabolism), Bacterial Proteins (toxicity), Coleoptera (drug effects), Coleoptera (physiology), Endotoxins (metabolism), Endotoxins (toxicity), Feeding Behavior (drug effects), Genetic Vectors (metabolism), Genome, Plastid (MeSH), Hemolysin Proteins (metabolism), Hemolysin Proteins (toxicity), Larva (drug effects), Larva (physiology), Phenotype (MeSH), Plant Leaves (parasitology), Plants, Genetically Modified (MeSH), Plastids (metabolism), Populus (genetics), Populus (parasitology), Transformation, Genetic (MeSH).
MESH :
- chemical , metabolism : Bacterial Proteins, Endotoxins, Hemolysin Proteins.
- drug effects : Coleoptera, Feeding Behavior, Larva.
- genetics : Populus.
- metabolism : Bacillus thuringiensis, Genetic Vectors, Plastids.
- parasitology : Plant Leaves, Populus.
- physiology : Coleoptera, Larva.
- chemical , toxicity : Bacterial Proteins, Endotoxins, Hemolysin Proteins.
- Animals, Genome, Plastid, Phenotype, Plants, Genetically Modified, Transformation, Genetic.

Abstract

KEY MESSAGE

The Bacillus thuringiensis (Bt) cry3Bb gene was successfully introduced into poplar plastid genome, leading to transplastomic poplar with high mortality to Plagiodera versicolora. Poplar (Populus L.) is one of the main resource of woody industry, but being damaged by insect pests. The feasibility and efficiency of plastid transformation technology for controlling two lepidopteran caterpillars have been demonstrated previously. Here, we introduced B. thuringiensis (Bt) cry3Bb into poplar plastid genome by biolistic bombardment for controlling P. versicolora, a widely distributed forest pest. Chimeric cry3Bb gene is controlled by the tobacco plastid rRNA operon promoter combined with the 5'UTR from gene10 of bacteriophage T7 (NtPrrn:T7g10) and the 3'UTR from the E. coli ribosomal RNA operon rrnB (TrrnB). The integration of transgene and homoplasmy of transplastomic poplar plants was confirmed by Southern blot analysis. Northern blot analysis indicated that cry3Bb was transcribed to both read through and shorter length transcripts in plastid. The transplastomic poplar expressing Cry3Bb insecticidal protein showed the highest accumulation level in young leaves, which reach up to 16.8 μg/g fresh weight, and comparatively low levels in mature and old leaves. Feeding the young leaves from Bt-Cry3Bb plastid lines to P. versicolora caused 100% mortality in the first-instar larvae after only 1 day, in the second-instar larvae after 2 days, and in the third-instar larvae for 3 days. Thus, we report a successful extension of plastid engineering poplar against the chrysomelid beetle.

DOI: 10.1007/s00299-019-02492-0
PubMed: 31797051

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000580
to stream Main, to step Curation: 000580

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.</title>
<author><name sortKey="Xu, Shijing" sort="Xu, Shijing" uniqKey="Xu S" first="Shijing" last="Xu">Shijing Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Yiqiu" sort="Zhang, Yiqiu" uniqKey="Zhang Y" first="Yiqiu" last="Zhang">Yiqiu Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Shengchun" sort="Li, Shengchun" uniqKey="Li S" first="Shengchun" last="Li">Shengchun Li</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Pharmacy, Binzhou Medical University, Yantai, 264003</wicri:regionArea>
<wicri:noRegion>264003</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chang, Ling" sort="Chang, Ling" uniqKey="Chang L" first="Ling" last="Chang">Ling Chang</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Pharmacy, Binzhou Medical University, Yantai, 264003</wicri:regionArea>
<wicri:noRegion>264003</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wu, Yuyong" sort="Wu, Yuyong" uniqKey="Wu Y" first="Yuyong" last="Wu">Yuyong Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China. wuyuyong_820@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China. wuyuyong_820@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Pharmacy, Binzhou Medical University, Yantai, 264003</wicri:regionArea>
<wicri:noRegion>264003</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Jiang" sort="Zhang, Jiang" uniqKey="Zhang J" first="Jiang" last="Zhang">Jiang Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China. zhangjiang@hubu.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2020">2020</date>
<idno type="RBID">pubmed:31797051</idno>
<idno type="pmid">31797051</idno>
<idno type="doi">10.1007/s00299-019-02492-0</idno>
<idno type="wicri:Area/Main/Corpus">000580</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000580</idno>
<idno type="wicri:Area/Main/Curation">000580</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000580</idno>
<idno type="wicri:Area/Main/Exploration">000580</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.</title>
<author><name sortKey="Xu, Shijing" sort="Xu, Shijing" uniqKey="Xu S" first="Shijing" last="Xu">Shijing Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Yiqiu" sort="Zhang, Yiqiu" uniqKey="Zhang Y" first="Yiqiu" last="Zhang">Yiqiu Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Shengchun" sort="Li, Shengchun" uniqKey="Li S" first="Shengchun" last="Li">Shengchun Li</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Pharmacy, Binzhou Medical University, Yantai, 264003</wicri:regionArea>
<wicri:noRegion>264003</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chang, Ling" sort="Chang, Ling" uniqKey="Chang L" first="Ling" last="Chang">Ling Chang</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Pharmacy, Binzhou Medical University, Yantai, 264003</wicri:regionArea>
<wicri:noRegion>264003</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wu, Yuyong" sort="Wu, Yuyong" uniqKey="Wu Y" first="Yuyong" last="Wu">Yuyong Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China. wuyuyong_820@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China. wuyuyong_820@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Pharmacy, Binzhou Medical University, Yantai, 264003</wicri:regionArea>
<wicri:noRegion>264003</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Jiang" sort="Zhang, Jiang" uniqKey="Zhang J" first="Jiang" last="Zhang">Jiang Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China. zhangjiang@hubu.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062</wicri:regionArea>
<wicri:noRegion>430062</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Plant cell reports</title>
<idno type="eISSN">1432-203X</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Bacillus thuringiensis (metabolism)</term>
<term>Bacterial Proteins (metabolism)</term>
<term>Bacterial Proteins (toxicity)</term>
<term>Coleoptera (drug effects)</term>
<term>Coleoptera (physiology)</term>
<term>Endotoxins (metabolism)</term>
<term>Endotoxins (toxicity)</term>
<term>Feeding Behavior (drug effects)</term>
<term>Genetic Vectors (metabolism)</term>
<term>Genome, Plastid (MeSH)</term>
<term>Hemolysin Proteins (metabolism)</term>
<term>Hemolysin Proteins (toxicity)</term>
<term>Larva (drug effects)</term>
<term>Larva (physiology)</term>
<term>Phenotype (MeSH)</term>
<term>Plant Leaves (parasitology)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Plastids (metabolism)</term>
<term>Populus (genetics)</term>
<term>Populus (parasitology)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Bacillus thuringiensis (métabolisme)</term>
<term>Coléoptères (effets des médicaments et des substances chimiques)</term>
<term>Coléoptères (physiologie)</term>
<term>Comportement alimentaire (effets des médicaments et des substances chimiques)</term>
<term>Endotoxines (métabolisme)</term>
<term>Endotoxines (toxicité)</term>
<term>Feuilles de plante (parasitologie)</term>
<term>Génome plastidique (MeSH)</term>
<term>Hémolysines (métabolisme)</term>
<term>Hémolysines (toxicité)</term>
<term>Larve (effets des médicaments et des substances chimiques)</term>
<term>Larve (physiologie)</term>
<term>Phénotype (MeSH)</term>
<term>Plastes (métabolisme)</term>
<term>Populus (génétique)</term>
<term>Populus (parasitologie)</term>
<term>Protéines bactériennes (métabolisme)</term>
<term>Protéines bactériennes (toxicité)</term>
<term>Transformation génétique (MeSH)</term>
<term>Vecteurs génétiques (métabolisme)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term>
<term>Endotoxins</term>
<term>Hemolysin Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Coleoptera</term>
<term>Feeding Behavior</term>
<term>Larva</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Coléoptères</term>
<term>Comportement alimentaire</term>
<term>Larve</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillus thuringiensis</term>
<term>Genetic Vectors</term>
<term>Plastids</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bacillus thuringiensis</term>
<term>Endotoxines</term>
<term>Hémolysines</term>
<term>Plastes</term>
<term>Protéines bactériennes</term>
<term>Vecteurs génétiques</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Feuilles de plante</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Leaves</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coléoptères</term>
<term>Larve</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coleoptera</term>
<term>Larva</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Bacterial Proteins</term>
<term>Endotoxins</term>
<term>Hemolysin Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Endotoxines</term>
<term>Hémolysines</term>
<term>Protéines bactériennes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Genome, Plastid</term>
<term>Phenotype</term>
<term>Plants, Genetically Modified</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Génome plastidique</term>
<term>Phénotype</term>
<term>Transformation génétique</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><b>KEY MESSAGE</b>
</p>
<p>The Bacillus thuringiensis (Bt) cry3Bb gene was successfully introduced into poplar plastid genome, leading to transplastomic poplar with high mortality to Plagiodera versicolora. Poplar (Populus L.) is one of the main resource of woody industry, but being damaged by insect pests. The feasibility and efficiency of plastid transformation technology for controlling two lepidopteran caterpillars have been demonstrated previously. Here, we introduced B. thuringiensis (Bt) cry3Bb into poplar plastid genome by biolistic bombardment for controlling P. versicolora, a widely distributed forest pest. Chimeric cry3Bb gene is controlled by the tobacco plastid rRNA operon promoter combined with the 5'UTR from gene10 of bacteriophage T7 (NtPrrn:T7g10) and the 3'UTR from the E. coli ribosomal RNA operon rrnB (TrrnB). The integration of transgene and homoplasmy of transplastomic poplar plants was confirmed by Southern blot analysis. Northern blot analysis indicated that cry3Bb was transcribed to both read through and shorter length transcripts in plastid. The transplastomic poplar expressing Cry3Bb insecticidal protein showed the highest accumulation level in young leaves, which reach up to 16.8 μg/g fresh weight, and comparatively low levels in mature and old leaves. Feeding the young leaves from Bt-Cry3Bb plastid lines to P. versicolora caused 100% mortality in the first-instar larvae after only 1 day, in the second-instar larvae after 2 days, and in the third-instar larvae for 3 days. Thus, we report a successful extension of plastid engineering poplar against the chrysomelid beetle.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31797051</PMID>
<DateCompleted><Year>2020</Year>
<Month>09</Month>
<Day>17</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>30</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>39</Volume>
<Issue>3</Issue>
<PubDate><Year>2020</Year>
<Month>Mar</Month>
</PubDate>
</JournalIssue>
<Title>Plant cell reports</Title>
<ISOAbbreviation>Plant Cell Rep</ISOAbbreviation>
</Journal>
<ArticleTitle>Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.</ArticleTitle>
<Pagination><MedlinePgn>317-323</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s00299-019-02492-0</ELocationID>
<Abstract><AbstractText Label="KEY MESSAGE" NlmCategory="UNASSIGNED">The Bacillus thuringiensis (Bt) cry3Bb gene was successfully introduced into poplar plastid genome, leading to transplastomic poplar with high mortality to Plagiodera versicolora. Poplar (Populus L.) is one of the main resource of woody industry, but being damaged by insect pests. The feasibility and efficiency of plastid transformation technology for controlling two lepidopteran caterpillars have been demonstrated previously. Here, we introduced B. thuringiensis (Bt) cry3Bb into poplar plastid genome by biolistic bombardment for controlling P. versicolora, a widely distributed forest pest. Chimeric cry3Bb gene is controlled by the tobacco plastid rRNA operon promoter combined with the 5'UTR from gene10 of bacteriophage T7 (NtPrrn:T7g10) and the 3'UTR from the E. coli ribosomal RNA operon rrnB (TrrnB). The integration of transgene and homoplasmy of transplastomic poplar plants was confirmed by Southern blot analysis. Northern blot analysis indicated that cry3Bb was transcribed to both read through and shorter length transcripts in plastid. The transplastomic poplar expressing Cry3Bb insecticidal protein showed the highest accumulation level in young leaves, which reach up to 16.8 μg/g fresh weight, and comparatively low levels in mature and old leaves. Feeding the young leaves from Bt-Cry3Bb plastid lines to P. versicolora caused 100% mortality in the first-instar larvae after only 1 day, in the second-instar larvae after 2 days, and in the third-instar larvae for 3 days. Thus, we report a successful extension of plastid engineering poplar against the chrysomelid beetle.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Shijing</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Yiqiu</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Shengchun</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chang</LastName>
<ForeName>Ling</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Yuyong</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China. wuyuyong_820@126.com.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>School of Pharmacy, Binzhou Medical University, Yantai, 264003, China. wuyuyong_820@126.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Jiang</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0003-3949-3765</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China. zhangjiang@hubu.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>2017YFD0600101</GrantID>
<Agency>National Key Research and Development Program of China</Agency>
<Country></Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>12</Month>
<Day>03</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Plant Cell Rep</MedlineTA>
<NlmUniqueID>9880970</NlmUniqueID>
<ISSNLinking>0721-7714</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004731">Endotoxins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006460">Hemolysin Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C030956">insecticidal crystal protein, Bacillus Thuringiensis</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001413" MajorTopicYN="N">Bacillus thuringiensis</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001517" MajorTopicYN="N">Coleoptera</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004731" MajorTopicYN="N">Endotoxins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005247" MajorTopicYN="Y">Feeding Behavior</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054627" MajorTopicYN="N">Genome, Plastid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006460" MajorTopicYN="N">Hemolysin Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007814" MajorTopicYN="N">Larva</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018087" MajorTopicYN="N">Plastids</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacillus thuringiensis</Keyword>
<Keyword MajorTopicYN="N">Insect resistance</Keyword>
<Keyword MajorTopicYN="N">Leaf beetle</Keyword>
<Keyword MajorTopicYN="N">Plastid genome engineering</Keyword>
<Keyword MajorTopicYN="N">Poplar</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year>
<Month>09</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>11</Month>
<Day>13</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>12</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>9</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>12</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">31797051</ArticleId>
<ArticleId IdType="doi">10.1007/s00299-019-02492-0</ArticleId>
<ArticleId IdType="pii">10.1007/s00299-019-02492-0</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Folia Microbiol (Praha). 2013 May;58(3):201-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23054688</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2019 Jun;249(6):1963-1975</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30900084</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2003 Dec;21(12):1493-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14608363</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2001 Feb;25(3):261-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11208018</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Elife. 2016 Jun 14;5:</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27296645</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):E623-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23382222</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Insect Biochem Mol Biol. 2014 Feb;45:11-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24225445</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Biotechnol. 2001 Dec;19(12):500-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11711193</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2012 Jul;78(14):4795-801</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22544259</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2012 Oct;72(1):115-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22639905</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Jan;131(1):177-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12529526</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Entomol. 1998;43:701-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15012402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2011 Jul;76(3-5):385-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20859755</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2017 Aug 21;8:1454</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28871270</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Tree Physiol. 2019 Sep 1;39(9):1525-1532</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31222266</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2011 Mar;39(4):1427-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20965967</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2009 May;75(10):3086-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19329664</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2017 Jan 13;12(1):e0170201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28085955</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Theor Appl Genet. 1992 Jul;84(3-4):280-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24203184</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2001 Jan;19(1):71-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11135556</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2015;66:211-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25494465</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Shijing" sort="Xu, Shijing" uniqKey="Xu S" first="Shijing" last="Xu">Shijing Xu</name>
</noRegion>
<name sortKey="Chang, Ling" sort="Chang, Ling" uniqKey="Chang L" first="Ling" last="Chang">Ling Chang</name>
<name sortKey="Chang, Ling" sort="Chang, Ling" uniqKey="Chang L" first="Ling" last="Chang">Ling Chang</name>
<name sortKey="Li, Shengchun" sort="Li, Shengchun" uniqKey="Li S" first="Shengchun" last="Li">Shengchun Li</name>
<name sortKey="Li, Shengchun" sort="Li, Shengchun" uniqKey="Li S" first="Shengchun" last="Li">Shengchun Li</name>
<name sortKey="Wu, Yuyong" sort="Wu, Yuyong" uniqKey="Wu Y" first="Yuyong" last="Wu">Yuyong Wu</name>
<name sortKey="Wu, Yuyong" sort="Wu, Yuyong" uniqKey="Wu Y" first="Yuyong" last="Wu">Yuyong Wu</name>
<name sortKey="Zhang, Jiang" sort="Zhang, Jiang" uniqKey="Zhang J" first="Jiang" last="Zhang">Jiang Zhang</name>
<name sortKey="Zhang, Yiqiu" sort="Zhang, Yiqiu" uniqKey="Zhang Y" first="Yiqiu" last="Zhang">Yiqiu Zhang</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000202 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000202 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:31797051
   |texte=   Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:31797051" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.

Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki